Ebook Info.

1. Introduction 2. Basic Calculations for Reagent Preparation 3. Commonly Used Equipment’s in Molecular Biology Laboratory.4. DNA Isolation from Fish Tissue 5. RNA Isolation from Fish Tissue 6. First Strand cDNA Synthesis by Reverse Transcriptase PCR.7. Isolation of Plasmid DNA 8. Quantification of Nucleic Acids 9. Principles of PCR Primer Designing 10. Polymerase Chain Reaction (PCR) 11. Agarose Gel Electrophoresis (AGE) 12. The Real-Time Polymerase Chain Reaction (RT-PCR) 13. Preparation of Competent Cells 14. T/A Cloning of PCR Products 15. Restriction Endonuclease Digestion 16. Separation of Proteins on SDS-PAGE 17. Southern Blotting Technique18. Northern Blotting Technique 19. Western Blotting Technique 20. Fish Cell Culture Technique 21. DNA Barcoding of Fishes 22. Single Cell Gel Electrophoresis (Comet Assay) 23. Methods of Chromosome Preparation from Fish 24. Germ Cell Isolation from Fish Gonad 25. Immunohistochemistry